Resource Type

Journal Article 36

Year

2024 1

2022 2

2021 3

2020 1

2019 2

2018 5

2017 3

2015 2

2014 3

2012 2

2011 1

2010 1

2009 5

2008 1

2007 3

1999 1

open ︾

Keywords

monoclonal antibody 2

recombinant human growth hormone 2

transgenic mice 2

yeast 2

21-gene assay 1

2b-BCG 1

4-fluorophenylpyruvate 1

Bacillus coagulans 1

Eospalax baileyi 1

Panthera pardus 1

Puccinellia tenuiflora 1

Amino acid analysis 1

Angiostrongylus cantonensis 1

Asp-AT 1

Assembly efficiency 1

BAC recombinant methods 1

Bioremediation 1

CCK-8 assay 1

Chinese hamster ovary (CHO) 1

open ︾

Search scope:

排序： Display mode:

In vitro agonistic and antagonistic endocrine disrupting effects of organic extracts from waste water of different treatment processes

Kaifeng RAO, Na LI, Mei MA, Zijian WANG

Frontiers of Environmental Science & Engineering 2014, Volume 8, Issue 1,   Pages 69-78 doi: 10.1007/s11783-013-0502-7

Abstract: In the present study, a battery of in vitro yeast bioassays and a cell bioassay, including antagonistic

Keywords： waste water     in vitro     recombinant yeast assay     ethoxyresorufin-O-deethylase (EROD)     receptor     endocrine    

HTML PDF Collect

Use of dry yeast cells as a cheap nitrogen source for lactic acid production by thermophilic

Kim Yng Ooi, Jin Chuan Wu

Frontiers of Chemical Science and Engineering 2015, Volume 9, Issue 3,   Pages 381-385 doi: 10.1007/s11705-015-1534-2

Abstract: Dry yeast cells (DYC) were used as a cheap nitrogen source to replace expensive yeast extract (YE) for

Keywords： L-lactic acid     thermophilic strain     Bacillus coagulans     dry yeast cells     autolysis     fermentation    

HTML PDF Collect

SCRaMbLE drive application of synthetic yeast genome

Jin Jin, Yuan Ma, Duo Liu

Frontiers of Chemical Science and Engineering 2018, Volume 12, Issue 4,   Pages 832-834 doi: 10.1007/s11705-018-1749-0

Abstract:
HTML PDF Collect

High-expression of recombinant human concensus interferon-α by Pichia pastoris

HAO Yuyou, SHI Qiqi, HE Yun, ZHUANG Yingping, WANG Yonghong, ZHANG Siliang, CHU Ju, LIU Zhimin

Frontiers of Chemical Science and Engineering 2007, Volume 1, Issue 4,   Pages 399-403 doi: 10.1007/s11705-007-0073-x

Abstract: The present work focused on the high expression of recombinant human consensus interferon- (cIFN) by

Keywords： bioactivity     pastoris     maximum     induction     optimized    

HTML PDF Collect

Biosensor-based assay of exosome biomarker for early diagnosis of cancer

Frontiers of Medicine 2022, Volume 16, Issue 2,   Pages 157-175 doi: 10.1007/s11684-021-0884-z

Abstract: In this review, the latest advances of biosensors regarding the assay of exosomes were summarized, and

Keywords： biosensor     exosome     cancer diagnosis    

HTML PDF Collect

Expression and function of DMT1 without IRE in C6 cells mediated by recombinant adenovirus

Xixun DU*, Huamin XU*, Hong JIANG, Jun WANG, Lei WANG, Junxia XIE

Frontiers of Medicine 2009, Volume 3, Issue 1,   Pages 67-71 doi: 10.1007/s11684-009-0010-0

Abstract: In the present study, we constructed a recombinant adenovirus containing the gene of DMT1 without theIRE was then transfected into E1-transformed human embryonic kidney cells (HEK293 cells) , in which recombinantPCR confirmed pAdEasy1-DMT1-IRE contained gene DMT1-IRE, indicating the successful construction of recombinant

Keywords： divalent metal transporter 1     recombinant adenovirus     homologous recombination     iron    

HTML PDF Collect

Construction of a universal recombinant expression vector that regulates the expression of human lysozyme

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 3,   Pages 382-389 doi: 10.15302/J-FASE-2018211

Abstract:

The mammary gland provides a novel method for producing recombinant proteins in milk of transgenicpronuclei of fertilized mouse embryos, to successfully generate two transgenic mouse lines that expressed recombinant

Keywords： BAC recombinant methods     gene expression     human lysozyme     transgenic mice     milk expression vector    

HTML PDF Collect

Recombinant protein diannexin prevents preeclampsia-like symptoms in a pregnant mouse model via reducing

Frontiers of Medicine 2022, Volume 16, Issue 6,   Pages 919-931 doi: 10.1007/s11684-021-0918-6

Abstract: This study describes a strategy to prevent PE by reducing MP release using the recombinant protein, diannexin

Keywords： preeclampsia     recombinant protein diannexin     microparticle     NLRP3 inflammasome     phosphatidylserin    

HTML PDF Collect

Clinical evaluation of recombinant human growth hormone injection in children with growth hormone deficiency

Ling HOU, Xiaoping LUO, Minlian DU, Huamei MA, Chunxiu GONG, Yuchuan LI, Shuixian SHEN, Zhuhui ZHAO, Li LIANG, Guanping DONG, Chaoying YAN, Hongwei DU

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 171-176 doi: 10.1007/s11684-009-0027-4

Abstract: Recombinant human growth hormone (rhGH) has been widely used in the clinical treatment of growth hormone

Keywords： recombinant human growth hormone     injection     growth hormone deficiency    

HTML PDF Collect

supplementation of thyroid hormone on treatment of idiopathic short-stature children during therapy with recombinant

Wei Wang, Shuqin Jiang, Zhirui Cui, Xiangyang Luo, Lingli Shi, Heli Zheng

Frontiers of Medicine 2018, Volume 12, Issue 5,   Pages 580-585 doi: 10.1007/s11684-017-0585-9

Abstract: deficiency on growth rate should be considered when FT4 level lies in the low-normal range prior to recombinant

Keywords： therapeutic     idiopathic short-stature children     free T4     the first year     recombinant human growth hormone    

HTML PDF Collect

Clinical outcomes of ATP-tumor chemosensitivity assay directed chemotherapy in hepatocellular carcinoma

RAO Rongsheng, CHEN Wenxue, ZHOU Xinwen, ZHOU Zheng, LI Xiaojun, ZENG Zhiping

Frontiers of Medicine 2007, Volume 1, Issue 2,   Pages 157-160 doi: 10.1007/s11684-007-0029-z

Abstract: This study aims to investigate the clinical response of ATP tumor chemosensitivity assay (ATP-TCA) directedThis tissue was put through the assay to determine chemosensitivity.
HTML PDF Collect

Cloning of human XAF1 gene promoter and assay of its transcription activity in a variety of cell lines

Qiong CHEN, Qing YU, Yuhu SONG, Peiyuan Li, Ying CHANG, Zhijun WANG, Lifeng LIU, Wei WU, Jusheng LIN

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 148-152 doi: 10.1007/s11684-009-0032-7

Abstract: amplified by polymerase chain reaction (PCR) and cloned into pGL3-basic vector and pEGFP-1 vector to assayThe promoter transcription activity was determined by dual-luciferase report assay, and enhanced greenThe dual-luciferase reporter assay showed that the promoter transcription activity in digestive system

Keywords： gene     X-linked inhibitor of apoptosis protein associated factor-1 (XAF1)     promoter     transcription regulation    

HTML PDF Collect

Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 179-184 doi: 10.15302/J-FASE-2014020

Abstract: Expression analysis showed that recombinant hBCHE (rhBCHE) was expressed efficiently in the mammary gland

Keywords： recombinant human butyrylcholinesterase (rhBCHE)     human lactoferrin bacterial artificial chromosome (hLF    

HTML PDF Collect

An extraction- assay system: Evaluation on flavonols in plant resistance to Pb and Cd by supercritical

Xu Zhang, Huanhuan Yang, Xinlei Wang, Wen Song, Zhaojie Cui

Frontiers of Environmental Science & Engineering 2018, Volume 12, Issue 4, doi: 10.1007/s11783-018-1059-2

Abstract:

SFE-GC system is established for flavonols assessment.

Optimal parameters of SFE-GC are evaluated and determined.

Quercetin and kaempferol are detected in plant under heavy metal stress.

Gene expression analysis shows consistent regularity with content of flavonols.

ROS level is applied for elaborating the plant resistance status.

Keywords： Flavonols     Heavy metal     Supercritical carbon dioxide extraction (SFE)     GC     Plant resistance    

HTML PDF Collect

High-level expression of recombinant IgG1 by CHO K1 platform

Ningning Xu, Jianfa Ou, Al-Karim (Al) Gilani, Lufang Zhou, Margaret Liu

Frontiers of Chemical Science and Engineering 2015, Volume 9, Issue 3,   Pages 376-380 doi: 10.1007/s11705-015-1531-5

Abstract: The Chinese Hamster Ovary (CHO K1) cell was used to express a targeted anti-cancer monoclonal antibody by optimizing the platform of the construction of production cell line in this study. The adherent CHO K1 was first adapted to suspension culture in chemical defined medium. Then the glutamine synthetase (GS) vector was applied to construct a single plasmid to overexpress a monoclonal antibody IgG1. Post transfection, the production of cell pool was optimized by glutamine-free selection and amplification using various concentrations of methionine sulfoximine. The best cell pool of CHO K1/IgG1 was used to screen the top single clone using the limiting dilution cloning. Finally, a high IgG1 production of 780 mg/L was obtained from a batch culture. This study demonstrated that the construction of high producing cell line, from gene to clone, could be completed within six month and the gene amplification improved protein production greatly.

Keywords： Chinese hamster ovary (CHO)     monoclonal antibody     IgG1     amplification     cell line development    

HTML PDF Collect

Title Author Date Type Operation

In vitro agonistic and antagonistic endocrine disrupting effects of organic extracts from waste water of different treatment processes

Kaifeng RAO, Na LI, Mei MA, Zijian WANG

Journal Article

Use of dry yeast cells as a cheap nitrogen source for lactic acid production by thermophilic

Kim Yng Ooi, Jin Chuan Wu

Journal Article

SCRaMbLE drive application of synthetic yeast genome

Jin Jin, Yuan Ma, Duo Liu

Journal Article

High-expression of recombinant human concensus interferon-α by Pichia pastoris

HAO Yuyou, SHI Qiqi, HE Yun, ZHUANG Yingping, WANG Yonghong, ZHANG Siliang, CHU Ju, LIU Zhimin

Journal Article

Biosensor-based assay of exosome biomarker for early diagnosis of cancer

Journal Article

Expression and function of DMT1 without IRE in C6 cells mediated by recombinant adenovirus

Xixun DU*, Huamin XU*, Hong JIANG, Jun WANG, Lei WANG, Junxia XIE

Journal Article

Construction of a universal recombinant expression vector that regulates the expression of human lysozyme

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Journal Article

Recombinant protein diannexin prevents preeclampsia-like symptoms in a pregnant mouse model via reducing

Journal Article

Clinical evaluation of recombinant human growth hormone injection in children with growth hormone deficiency

Ling HOU, Xiaoping LUO, Minlian DU, Huamei MA, Chunxiu GONG, Yuchuan LI, Shuixian SHEN, Zhuhui ZHAO, Li LIANG, Guanping DONG, Chaoying YAN, Hongwei DU

Journal Article

supplementation of thyroid hormone on treatment of idiopathic short-stature children during therapy with recombinant

Wei Wang, Shuqin Jiang, Zhirui Cui, Xiangyang Luo, Lingli Shi, Heli Zheng

Journal Article

Clinical outcomes of ATP-tumor chemosensitivity assay directed chemotherapy in hepatocellular carcinoma

RAO Rongsheng, CHEN Wenxue, ZHOU Xinwen, ZHOU Zheng, LI Xiaojun, ZENG Zhiping

Journal Article

Cloning of human XAF1 gene promoter and assay of its transcription activity in a variety of cell lines

Qiong CHEN, Qing YU, Yuhu SONG, Peiyuan Li, Ying CHANG, Zhijun WANG, Lifeng LIU, Wei WU, Jusheng LIN

Journal Article

Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Journal Article

An extraction- assay system: Evaluation on flavonols in plant resistance to Pb and Cd by supercritical

Xu Zhang, Huanhuan Yang, Xinlei Wang, Wen Song, Zhaojie Cui

Journal Article

High-level expression of recombinant IgG1 by CHO K1 platform

Ningning Xu, Jianfa Ou, Al-Karim (Al) Gilani, Lufang Zhou, Margaret Liu

Journal Article